Effect of sodium dodecyl benzene sulfonate on biofilm of Staphylococcus epidermidis with infection of the implant

Abstract:

[Background] With the widespread use of medical implants, hospital-acquired infections caused by Staphylococcus epidermidis biofilm are increasing. For now, there are few reports about the effect of surfactant on the biofilm of Staphylococcus epidermidis. [Objective] To demonstrate the effect of anionic surfactant sodium dodecyl benzene sulfonate, including scavenging ATCC 35984 biofilm, bacterial metabolism and formation of polysaccharide intercellular adhesion (PIA). To provide reliable theory and practical evidence for SDBS, and prevent related infections caused by Staphylococcus epidermidis biofilm in clinic. [Methods] XTT reduction method was used to explore the effect of SDBS on the clearance of ATCC 35984 biofilm and bacterial metabolism in the biofilm. Laser scanning confocal microscope (LSCM) and Congo red medium was respectively used to observe the effect of SDBS on biofilm and PIA. [Results] SDBS on the concentration of 256 mg/L, 128 mg/L, 64 mg/L, 32 mg/L, 16 mg/L has significantly clearance on ATCC 35984 in the 6 h, 12 h, 24 h, respectively (P<0.01). It is significantly inhibition effect of SDBS with 32 mg/L on bacterial metabolism in the biofilm (P<0.05). 256 mg/L, 128 mg/L, 64 mg/L SDBS has satisfied clearance. 64 mg/L and 32 mg/L SDBS has no inhibition effect on PIA formation under the LSCM observation. [Conclusion] SDBS has significant inhibition effect on bacterial metabolism in the biofilm of Staphylococus epidermidis and damage the morphological structure of biofilm.

Key words:Sodium dodecyl benzene sulfonate, Staphylococus epidermidis, biofilm, clearance, metabolism, polysaccharide intercellular adhesion