Advances in gene editing of Pichia pastoris

Abstract:

Pichia pastoris is a widely used host for recombinant protein expression. As a basic tool for metabolic engineering, gene editing technology exhibits great importance for the metabolic modification of Pichia pastoris. In the past decade, gene editing technologies have developed rapidly. Many new techniques, such as ZFN, TALEN and CRISPR/Cas9, were developed besides homologous recombination and Cre/loxP recombination which makes gene editing in P. pastoris more convenient and efficient. This review summarized the principle, application and research progress of gene editing techniques in Pichia pastoris. This paper also prospected the future development of gene editing in P. pastoris based on the advances of related fields.