Isolation, identification and yield correlation of cellulose-producing strains

Abstract:

[Background] Bacterial cellulose is a new type of natural bio-nanomaterials with excellent properties, but it has not yet been achieved production and application in large-scale because of the low fermentation yield and high production cost. [Objective] We naturally selected and bred cellulose high-yield strains, and explored the relationship between the strain yield, colony morphology, fruits source and strains species. [Methods] Naturally bred cellulose-producing strains were selected from a total of 576 rotten fruits covering 15 species, and classified by colony morphologies. High-yield cellulose strains were screened by static culture, and the 16S rRNA gene region of the strains was sequenced to identify their species. [Results] 134 cellulose-producing strains were obtained. The best strain Komagataeibacter hansenii was isolated from mango with the yield 11.24 g/L. All strains was classified into 10 categories according to the colony morphology. The common characteristics of high-yield cellulose colonial morphologies were yellow, round, raised, neat or irregular edges and rough or wrinkled surface (morphology 4, 5, 9). The colonies’ morphological diversity of strains isolated from apple and pear were abundant. Most of high-yield cellulose strains were mango-derived, followed by pears and apples. All strains were identified as 5 genera and 13 species, including Acetobacter, Komagataeibacter, Gluconacetobacter, Serratia, and Lactobacillus, in which highly productive cellulose strains were concentrated in K. hansenii and K. intermedius. [Conclusion] The screened strains are rich in diversity, and a number of high-yield cellulose strains are obtained, which have good heredity stability and greatly enrich the source of bacterial cellulose production strains. Analysis of the relationship between the yield of the obtained strains with the colony morphology and fruit source may offers reference for future screening work.