miR-34b regulates the replication of Enterovirus 71 in rhabdomyosarcoma cell

Abstract:

[Background] MicroRNAs (miRNAs) play an important role in the process of infection and replication of virus in host cells. [Objective] In this study, we examined miR-34b effect on the replication of Enterovirus 71 in RD (rhabdomyosarcoma) cells. [Methods] Western blot and real-time PCR test were performed to investigate the effect of miRNAs on viral replication. Furthermore, dual-luciferase reporter assay was used to detect the interaction between miR-34b and potential target eIF4E, and the expression of eIF4E at mRNA levels was tested by real-time PCR. [Results] The study showed that miR-34b can stimulate the replication of EV71 virus. On the contrary, miR-34b inhibitor can suppress EV71 virus replication. Cellular miR-34b can regulate the replication of EV71 virus through binding to eIF4E in host cells. [Conclusion] Our paper should report the role of miR-34b in this regulation for the first time. Our findings support the notion that the cellular miRNAs play an important role in the host and virus infection.