High throughput sequencing analysis of bacterial communities in Yumin safflower

Abstract:

[Background] Xinjiang Yumin county is the largest safflower planting base of China, its unique geographical environment and climatic conditions suitable for the growth and it is beneficial to formation of quality of safflower. [Objective] The purpose of this study was to explore the relationship between the bacterial community of safflower rhizosphere soil and its environment factors in different growth periods in Xinjiang Yumin hilly area, which may help to provide scientific basis for revealing the nature and low of geoherbalism to genuine regional drugs. [Methods] Took the rhizosphere soil and bulk soil of the vegetative growth stage and reproductive growth stage in the 3 sample plots of Yumin cultivated safflower respectively, determined the soil properties and extracted the genomic DNA, and then established libraries through PCR amplification of 16S rRNA V4 region. Abundance, diversity and structure of soil bacteria were analyzed by bioinformatics technology after sequenced by Illumina HiSeq high-throughput sequencing platform. [Results] A total of 10 303 operational taxonomic units (OTUs) were obtained from 36 soil samples of safflower, and it have identified belonging to 405 species, 738 genera, 381 families, 201 orders, 102 classes, 47 phyla, in which the Actinobacteria (32.9%) and Proteobacteria (28.7%) were predominant. ANOVA analysis showed that there was no significant difference between the richness index Chao1 and the diversity index Shannon in the bacterial communities of the four group samples. However, the principal coordinate analysis (PCoA) and the distribution of species abundances at the level of bacterial genus revealed that there were significant differences in the composition of bacteria communities between different sample plots. [Conclusion] There are a lot to adapt to the changing environment of bacteria in rhizosphere soil of safflower, the presence of these bacteria may have positive effects on forming geoherbalism of the safflower. In addition, soil properties, plant growth stage and root exudates are the possible factors who committed to form the geoherbalism of safflower by effects the rhizosphere bacterial community.