Isolation of hypouricemic probiotics and exploration their effects on hyperuricemic rats

Abstract:

[Background] Hyperuricemia is well-known as a kind of metabolic disease with abnormal higher serum uric acid level. It is becoming a new method for treating hyperuricemia by degrading exogenous purine components ingested from food through probiotics. [Objective] Thirty probiotic strains of thirteen species were investigated for their abilities of lowering serum uric acid, we preliminarily propose the mechanism of its effects on hyperuricemic rats. [Methods] The ability of degrading nucleotides (adenosine monophosphate, guanosine monophosphate) , nucleosides (adenosine, guanosine), purines (xanthine, hypoxanthine and guanine) and uric acid by different probiotics were determined with HPLC, the probiotic strain with the fastest degrading ability was selected as the candidate strain for further test on hyperuricemic rats. Metabolites in the process of degrading nucleosides and nucleotides by probiotics were detected by mass, the mechanism of probiotics lowering the blood uric acid was preliminarily explored. [Results] Lactobacillus casei ZM15 was selected with the highest degradation rates of nucleosides and nucleotides. The animal experimental results showed that ZM15 had effects on hyperuricemia. Mass spectrometry results showed that after degradation of nucleotides and nucleotides in cell, guanine, xanthine and hypoxanthine appeared both inside and outside ZM15 cells, three kinds of purine content were markedly higher than those in normal cells, uric acid and allantoin were not found both inside and outside. [Conclusion] Lactobacillus casei ZM15 has the fasted degradation ability of nucleotide and nucleotide, and it has obvious effects of lowering uric acid on hyperuricemic model rats by mechanism of competition.