Microbial community structure and diversity in waterlogged wood and lacquer named F446 by Illumina MiSeq technology

Abstract:

[Background] The wood lacquer is vulnerable to microbial corrosion in the environment of water preserved wood lacquer because of microorganisms. [Objective] In order to study the microbial community structure and microbial disease information of wood lacquer ware. [Methods] The community structure of bacteria in wood lacquer and water samples was analyzed by Illumina MiSeq high throughput sequencing technology. [Results] There were differences between wood samples and water samples in the microbial community structure on the gate level, wood samples of a total of 7 dominant bacteria (relative abundance, gate>1%) were Proteobacteria (64.00%), Acidobacteria (14.70%) and Actinobacteria (3.83%). There were 6 dominant phyla detected at the phylum level, Proteobacteria (61.26%), Acidobacteria (8.25%) and Planctomycetes (4.88%), respectively. A total of 8 dominant species (relative abundance>1%) were found in the woody samples, which were Phenylobacterium (16.24%), Acidobacteria-Gp6 (9.68%) and Rhodoplanes (6.45%), respectively. There were 10 dominant genera in water samples, which were Naxibacter (9.03%), Acidobacteria-Gp6 (3.84%) and Nevskia (3.27%), respectively. The unclassified bacteria in wood samples accounted for 8.70% and 50.68%, respectively. The unclassified bacteria in water samples accounted for 12.83% and 59.35% respectively in the door and genus. [Conclusion] The microbial community structure of wood samples and water samples is abundant in the water preservation environment of wood lacquerware, but the microbial diversity is more complex than that of wood samples in the water samples of the door and genus level. In addition, a large number of potential new bacteria were detected in the tested samples.