Characterization of a phosphorus-solubilizing alkaline phosphatase AP3 cloned from Bacillus amyloliquefaciens YP6

Abstract:

[Background] The mechanisms of solubilizing-phosphorus by organisms are diverse, which significantly stimulates plant growths. [Objective] To characterize a phosphorus-solubilizing alkaline phosphatase from Bacillus amyloliquefaciens YP6. [Methods] An alkaline phosphatase, AP3, of YP6 was cloned, expressed in Escherichia coli BL21(DE3), characterized and its phosphorus-solubilizing effect tested. [Results] AP3 was an alkaline phosphatase, with optimal pH at 10.3 and optimal temperature at 40 °C. Vm was 4 033.4 μmol/(min·mg) and Km was 12.2 mmol/L. The soluble phosphate of rock phosphorus was enhanced obviously by enzyme AP3 treatment for 24 h, and by inoculation of the strain Bacillus amyloliquefaciens YP6 for 7 days. [Conclusion] Alkaline phosphatase AP3 can be used as a phosphorus-solubilizing enzyme.