Culturable microorganisms associated with basidiocarps of Thelephora ganbajun

Abstract:

[Background] Microorganisms have a role in spore germination, hyphal growth, mycorrhiza formation and basidiocarp development of mycorrhizal fungi. [Objective] Microorganisms of eight basidiocarps of Thelephora ganbajun which were collected from Songming County, Kunming and Lufeng County, Chuxiong Yi autonomous Prefecture, Yunnan Province, SW China was studied, which lay a foundation for follow-up studies on the interaction between microorganisms and T. ganbajun. [Methods] The dilution plate method was carried out to obtain the microbial isolates from basidiocarps of T. ganbajun. The difference of microbial quantity of T. ganbajun which were collected from different sites was analyzed by t-test. Diversity of microorganisms was analyzed by 16S rRNA gene and ITS amplification, sequencing and establishment of phylogenetic tree. [Results] A total of 282 culturable bacterial isolates of eight basidiocarps from Songming County and Lufeng County belonged to 15 species and 12 genera in 2 phyla. 80% of bacteria were affiliated to the Proteobacteria and dominated by γ-Proteobacteria and Pseudomonas. The other 20% were affiliated to the Bacteroidetes. A total of 114 culturable fungal isolates belonged to 10 species and 10 genera in 2 phyla. 62% of fungi were affiliated to the Ascomycota, dominated by Lophiostoma which was only isolated from Lufeng County. 38% of fungi were affiliated to the Basidiomycota, dominated by Asterotremella. [Conclusion] There was no significant difference in the number of CFU of bacteria (p=0.22) and fungi (p=0.65) among basidiocarps of T. ganbajun which were collected from different sites. All bacteria isolates were dominated by γ-Proteobacteria and Psedomonas. Fungi were dominated by Basidiomycota and Asterotremella in Songming, whereas fungi were dominated by Ascomycota and Lophiostoma in Lufeng.