Isolation, identification and multilocus sequence typing of Campylobacter from animal source in Anhui province

Abstract:

[Background] Campylobacter is a Gram-negative microaerobic bacterium. It is a major foodborne pathogen and causes human gastroenteritis worldwide. [Objective] This study was to isolate, identify and molecular type Campylobacter from animal in six different areas of Anhui province. [Methods] The strains were identified by morphological and culture characteristics, biochemical tests and PCR. Seven Campylobacter housekeeping genes including aspA, glnA, gltA, glyA, pgm, tkt and uncA were amplified by PCR and sequenced, and then the sequences of genes were analyzed using multilocus sequence typing (MLST) database and evolutionary tree. [Results] There were a total of 42 strains of Campylobacter were isolated from six areas. The isolates had more consistent morphological characteristics and similar biochemical properties. MLST results show that 32 sequence types, 9 new sequence types (including 8190, 8222, 8223, 8831, 8833, 8841, 8832, 8834 and 8843) and 6 new allelic profiles (including glnA606, glnA607, gltA518, glyA680, pgm863 and uncA541) were found. Evolutionary tree results show that C. jejuni and C. coli genetic relationship is very different and belong to two groups, which have five branches and three branches, respectively. [Conclusion] C. jejuni and C. coli have rich genotypes in Anhui province, and there is no obvious dominant genotype. From the perspective of genetic variation, C. jejuni is more complex and diverse, while C. coli is relatively conservative.