Isolation and bioactive characters of a novel lectin SHL24 from the liquid fermentation of Phellinus baumii

Abstract:

[Background] Phellinus baumii is a fungus with a high medicinal value and wide application prospect. The main active ingredient of Phellinus baumii is polysaccharide. Besides, lectin is another physiologically active substance which has the functions of immunomodulation, anti-tumor and antibacterial. [Objective] A novel lectin, SHL24, is isolated and purigied from the liquid fermentation of medicinal fungus Phellinus baumii and its bioactivity is studied. [Methods] Sephadex G-50, DEAE Sephadex A-25, MPLC-MonoQ and LPLC-Sephadex G-75 were used to purify this lectin. The molecular mass was determined by SDS-PAGE and LPLC-Sephadex G-75. The effects of different pH, metal ions, sugars, fermentation times, red blood cells, and temperatures on coagulation activity were studied. [Results] SHL24 is a single-subunit protein with the relative molecular weight of 24 kD. SHL24 can agglutinate red blood cells of mouse and human, while it has a variety of activities for different resources of red blood cells. SHL24 cannot be inhibited by D-mannose, D-maltose, dextrose, cane sugars, D-lactose, arabinose and L-rhamnose. SHL24 has good thermal stability and its coagulation activity isn’t inhibited by cations, such as Ca2+, Mg2+ and Zn2+. [Conclusion] The stable physical-chemical properties and biological activity of SHL24 make it worth to further study on its pharmacological action.