Research methods and trend of phylogenetic evolution of endophytic diazotrophs

Abstract:

To study phylogenetic relationships of endophytic nitrogen fixing bacteria in plants, common methods include morphology and protein level identification, numerical classification and automatic identification, chemical classification and identification, and molecular genetic identification. This paper introduces the key techniques of common methods, and discusses their advantages and disadvantages. With the development of high-throughput DNA sequencing technology in the field of microbiology, the whole genome sequencing has been applied to the study of microbial phylogenetic evolution. However, there is no systematic review of nitrogen-fixing endophytes whose genomes have been sequenced. A preliminary study on measuring whole genome sequences of endophytic diazotrophs. Several representative new methods based on genomic data (ANI analysis, the maximal unique match index, core genome analysis, component vector method, gene flow analysis) were summarized. Based on the current research methods of phylogenetic evolution, research trends of the development and evolution of Endophytic Nitrogen Fixing Bacteria were summarized and prospected. The purpose of this paper is to make the phylogenetic evolution of Endophytic Nitrogen Fixing Bacteria more accuracy and reliability.

Key words:Endophytic diazotrophs, Phylogenetic evolution, High-throughput sequencing, Genome analysis methods