Rapid acquisition of complete viral genome sequence of human adenovirus type 55

Abstract:

[Objective] To build a simple method for rapidly obtaining the full-length genome sequence of adenovirus type 55 (HAdV-55). [Methods] 12 pairs of primers were designed which covered the whole genome sequence of HAdV-55. HAdV-55 DNA was used as the template for PCR and twelve PCR products were obtained, which were sequenced respectively. Resulting sequences were assembled together and then the whole gene sequence was obtained. [Results] A strain of HAdV-55 (SF04/SC/2016) was isolated from throat swab specimens of a patient with acute upper respiratory tract infection. Twelve PCR products and their sequences were obtained. The sequences were assembled which yield the full-length genomic sequence. Sequence analysis was performed between this HAdV-55 strain and previous reported HAdV-55 virus in China. Phylogenetic tree was constructed by neighbor-joining method and this virus is in the same branch with previous reported HAdV-55 strains, which confirmed that this pathogen is HAdV-55. [Conclusion] The primer sequences and methods published in this study could facilitate the rapid sequencing of adenovirus, which will be contribute to reveal the evolutionary characteristics of HAdV-55 and the prevention and control of adenovirus type 55 related disease.

Key words:Adenovirus type 55, pathogen isolation, whole genome sequencing, sequence assembly