Intestinal microbiota in Li cohort and its correlation with their diets

Abstract:

[Objective] To analyze the intestinal microbiota of healthy Li cohort in Baisha of Hainan Province, study the profiles of intestinal microbiota of Li cohort and its correlation with their diet. [Methods] Morning faeces of 22 volunteers in Baisha of Hainan Province were selected as the study object, high-throughput sequencing technology based on 16S rRNA gene V3–V4 variable region was used to research the intestinal microbiota of Li cohort. Then, compared and analyzed the intestinal microbiota between Li cohort and other ethnic groups. At the same time, the nutrient intake of 22?volunteers of Li cohort was recorded in detail to study the profiles in intestinal microbiota of Li cohort and its correlation with their diet. [Results] Bacteroidetes (58.96%) and Firmicutes (37.77%) were the most abundant phylum in the gut of Li cohort. Meanwhile, at the genus level, Prevotella (49.38%) was the predominant genus. We compared the differences in gut microbiota between the Li cohort and other ethnic groups in China. Based on the analysis of α and β diversity in microbiota communities, the gut microbiota of Li cohort was significantly differences with other ethnic groups in China, and the α diversity in Li cohort was significantly lower than other ethnic groups. And the structure difference could be attributed to the genera of Catenibacterium, Prevotella, Megasphaera, Megamonas, Phascolarctobacterium and Blautia. The correlation between core microbiota and nutrients showed that Faecalibacterium prausnitzii was significantly positively related to the intake of dietary fiber, Cu, Mg and Mn, and negatively related to the intake of fat and VB2, while the Lactobacillus rogosae was significantly positively related to the intake of dietary fiber, Zn and Fe, and negatively related to the intake of niacin. [Conclusion] Present research reflected the differences in the gut microbiota among different regions and ethnic groups, and provided a theoretical basis for further understanding the balance in host’s intestinal microecology.