Regulation of pyoluteorin (Plt) biosynthesis and gene expression by global control protein Crc in Pseudomonas protegens H78

Abstract:

[Objective] The aim of this study was to study the influence of Crc (Cataboliterepression control protein) on pyoluteorin (Plt) biosynthesis and gene expression in Pseudomonas protegens H78. [Methods] The crc gene in the H78 chromosome was knocked out by homologous recombination. Plt production of H78 strain and its mutant grown in KMB was measured by HPLC. The regulation of Crc on plt operon expression was assessed by the lacZ reporter analysis. [Results] Plt production was significantly reduced in H78Δcrc. Crc positively regulated the expression of plt biosynthetic operon at the overall level, the transcriptional level and the post-transcriptional level. [Conclusion] The global regulator Crc positively regulates Plt production and its operon expression.

Key words:Pseudomonas protegens H78, Crc, pyoluteorin (Plt), biosynthesis, Plt biosynthetic operon