Effects of canopy damage on soil CO2 fixation bacterial community structure in Xiaokeng forest farm

Abstract:

[Objective] We designed and did experiments to simulate canopy damage in Xiaokeng forest farm, and to analyze the impact of canopy openness and litter input on soil CO2 fixation bacteria community structure. [Methods] A manipulative field experiment was conducted to study the effects of four different forms: control plots, damage-removal and treatment, damage-retention and processing, and untreated-add foliage treatment. Soil CO2 fixation bacteria community structure was measured by MiSeq analyses after a year of canopy damage. [Results] Based on bioinformatics and statistical analyses, community richness of soil CO2 fixation bacteria decreased and diversity increased under the influence of litter inputs and canopy openness. Nitrosospira increased significantly and became the dominant population, whereas the original dominant group of Bradyrhizobium decreased. Principal component analysis shows that community structures of soil CO2 fixation bacteria were changed. [Conclusion] A large number of litter inputs and increased canopy openness improved diversity of soil CO2 fixation bacteria, but decreased their community richness, and affected their community structure.

Key words:cbbL gene, Litter input, CO2 fixation bacteria, Canopy openness, Community structure