Distribution of Rib like protein in three lactobacilli bacteria

Abstract:

[Objective] We studied the distribution and potential function of Rib protein, a large bacterial cell surface protein founded in Lactobacillus reuteri, in different lactobacilli strains. [Methods] Primers were designed according to the 5′ non-repeat regain sequence of rib gene in L. reuteri ATCC55730, to PCR rib gene and heterologous expression. Truncated Rib protein from L. reuteri was expressed in Escherichia coli. The recombinant protein was purified and used for polyclonal antibody preparation. Western blot was done in subcellular localization analyses of Rib and Rib like protein detection. [Results] Western blot showed that Rib protein mainly distributed in the L. reuteri bacterial cell wall. Both PCR and Western blot analyses showed that Rib like protein was detected in 11 lactobacilli strains with different size. [Conclusion] Rib like protein is a probable widely distributing cell surface protein in lactobacilli strains, and may play a role in the adhesion of these lactobacilli on the host gut.