Effect of additional chemicals on antimicrobial susceptibility of Vibrio parahaemolyticus

Abstract:

[Objective] Antibiotic resistance is a severe public health problem. Recently, the relationship between bacterial metabolic regulation and antibiotic efficacy has been proposed in several pathogens. In the present study, we explored the effect of adding additional chemicals on drug susceptibility of Vibrio parahaemolyticus. [Methods] We studied the phenotypic change and compared the survival of V. parahemolyticus after treatment with antibiotics and different chemicals. The experimental results were conformed with carbonylcyanide-p-chlorophenyl hydrazine (CCCP). [Results] These results indicated that specific chemicals effectively potentiate kanamycin to eliminate multidrug resistant V. parahemolyticus. Among them, the addition of exogenous glucose could significantly enhance the sterilization ability with subinhibitory concentrations of kanamycin, but without effect on other antibiotics. The enhancement of antimicrobial susceptibility of Vibrio parahaemolyticus caused by exogenous chemicals could be eliminated after treatment with CCCP. [Conclusion] The sensitivity of V. parahemolyticus to aminoglycosides can be improved by regulating the metabolism of bacterial cells. Therefore, we present a novel approach in fighting against multidrug resistant V. parahemolyticus with practical application value.