Isolation , identification and degradation characterization of an alkane-degrading Acinetobacter sp. LAM1007

Abstract:

[Objective] We isolated and identified alkane-degrading bacteria to provide microbial resources to remediate petroleum hydrocarbon pollution. [Methods] With n-hexadecane as sole carbon source, we identified strain LAM1007 isolated from Daqing oil-contaminated field by analysis of morphological, physiological and biochemical characteristics, cellular chemical composition, and 16S rRNA gene sequence. Furthermore, single factor experiment was used to evaluate various parameters including temperature, pH, biomass, shaking speed, concentration of n-hexadecane (0.1%, 0.3%, 0.5%, 1.0%, 1.5% and 2.0%, v/v). [Results] Strain LAM1007 was isolated and identified as a member of the genus Acinetobacter. Optimum condition of the strain was in an inorganic salt medium supplemented with n-hexadecane at 30 °C, pH 7.0, 1% (v/v) biomass and 180 r/min. The degradation of n-hexadecane (0.3% v/v) was 90% after 60 h under the optimum conditions. [Conclusion] Acinetobacter sp. LAM1007 has the potential to remediate petroleum hydrocarbon pollution.

Key words:n-Hexadecane, Acinetobacter sp., Diesel, Paraffin liquid