Diversity and community structure of gut actinobacteria of giant panda

Abstract:

[Objective] Study the diversity and community structure of gut actinobacteria of giant panda with the different age and gender, in order to provide scientific basis for searching the actinobacteria possessing potential to produce bioactive compounds. [Methods] Denaturing gradient gel electrophoresis (DGGE) was employed to reveal the gut actinobacteria diversity and community structure of giant panda, and the results were analyzed by multiple comparisons, including UPGMA cluster, PCA and biological diversity of gut actinobacteria in different age and gender. [Results] The diversity and community structure of gut actinobacteria in different panda exhibited significant differences by DGGE analysis. The Shannon-Wiener index (H') and Richness (S) of gut actinobacteria in female giant pandas decreased as they grew older, but the male giant pandas were opposite. The different diversity of gut actinobacteria were observed in different giant panda. However, the diversity index of gut actinobacteria from the same gender possessed higher similarity. The recovery of DGGE strip series showed the 16S rRNA gene sequence similarity between the 28 stronger bands and the typical strains published in GenBank was 96% to 100%, and those sequences belonged to ten genera, Streptomyces was dominant genera accounting for 46% of all sequences, the remainder belonged to genera Kitasatospora, Rhodococcus, Corynebacterium, Dietziaceae, Marmoricola, Beutenbergia, Microbacterium, Streptacidiphilus, Blastococcus, which account for 54%. [Conclusion] This result revealed the intestinal actinobacteria community structure and diversity of giant panda were different and abundant, which were influenced by their age and gender.