Identification of rhizosphere bacterial communities of Narcissus tazetta

Abstract:

[Objective] The study was conducted to identify the composition of rhizosphere bacterial communities of Narcissus tazetta and their interaction with growth properties of Narcissus. [Methods] Rhizosphere soil samples of healthy Chongming Narcisuss and Zhangzhou Narcisuss were collected separately. Then, 16S rRNA gene amplification and high-throughput sequencing were used to compare the rhizosphere bacterial composition and diversities of two samples. [Results] The main 12 categories of rhizosphere bacteria of Chongming Narcisuss and Zhangzhou Narcisuss are similar. Proteobacteria, Chloroflexi, Bacteroidetes, Acidobacteria are dominant in the rhizosphere soil of Zhangzhou Narcissus and Chongming Narcissus. However, specific rhizosphere bacterial communities were also found for Zhangzhou Narcissus and Chongming Narcissus based on Venn and PCA analyses. There are more Planctomycetales, Xanthomonadales in rhizosphere soil of Chongming Narcissus while more Chloroflexales, Chlorobiales of Zhangzhou Narcissus. [Conclusions] The composition of rhizosphere bacterial communities may be closely related to the growth properties. The diversity and richness of the rhizosphere bacterial, such as anaerobic ammonium oxidation, opportunistic pathogens and photosynthetic bacteria, could have a crucial influence on growth properties of Narcissus.