Teaching module for experimental practice on “screening of amylase-producing bacteria, optimization of fermentation conditions, bacterial identification, and strain improvement”

Abstract:

The implementation of the experimental research module into microbiology experiment teaching improves undergraduate students’ motivation and engagement with inquiry-based learning. In this experimental research module “screening of amylase producing bacteria, optimization of fermentation conditions, bacterial identification, and strain improvement”, students will focus on studies of amylase-producing bacteria, and will be assigned with 5 experimental units that include preparation of culture medium, collection of soil samples and isolation of amylase-producing bacteria, optimization of fermentation conditions of the isolated amylase-producing bacterium, morphologic and molecular identification of bacterial strain, and strain improvement through ultraviolet mutagenesis. This experimental research module will guide students to experience an entire inquiry-based learning process by consistently planning and conducting research projects, through which students will learn and eventually master fundamental microbial experimental skills. This module is student-centered, implemented with “fixed plus mobile” mode, to ensure its integrity and consistency. To improve students’ scientific literacy and basic microbiological techniques, the examination is composed of two parts: scientific paper writing and onsite skills test. Overall, this experimental research module inspires students’ enthusiasm and motivation in learning microbiology; meanwhile it improves the teaching quality of microbiology curriculums.