Identification of Colletotrichum species associated with Saxifraga stolonifera anthracnose

Abstract:

[Objective] The study aims to isolate and identify the pathogen from Saxifraga stolonifera anthracnose. [Methods] The pathogen were isolated by tissue associated anthracnose and confirmed through Koch’s postulates, then the strains were identified through morphologic characteristics and multi-locus (ACT, CAL, CHS-1, GAPDH, ITS, TUB2) phylogeny analysis. [Results] Two strains, isolated from the leaves of S. stolonifera, were numbered as LPSU 20120244 and LPSU 20120251. Conidium of both two strains was hyaline, aseptate, straight, cylindrical. Conidium size of LPSU 20120244 was (11?25) μm×(5?9) μm, LPSU 20120251, (15?25) μm×(5?7) μm. Strain LPSU 20120244 and LPSU 20120251 was clustered with Colletotrichum karstii Y.L. Yang, Zuo Y. Liu, K.D. Hyde & L. Cai and C. jiangxiense F. Liu & L. Cai, respectively, and the bootstrap support of both two clades were 100%. [Conclusion] Based on morphologic characteristics and multi-locus phylogeny analysis, the two strains represent 2 Colletotrichum species, Colletotrichum karstii (strain LPSU 20120244) and C. jiangxiense (strain LPSU 20120251).