Determination of bacteria diversity of degraded grassland in Xinyuan county by high-throughput sequencing technology

Abstract:

[Objective] In order to study the community diversity of degraded grassland caused by overgrazing, 6 soil samples collected from Xinyuan county were investigated. [Methods] Bacterial diversity and community structure of the samples were analyzed via high throughput sequencing technology Illumina HiSeq 2500. [Results] The mean value Chao1 and Shannon index were 2 118.27 and 8.84, respectively; The bacterial community mainly belong to 35 phylum including Actinobacteria, Proteobacteria, Firmicutes, Gemmatimonadetes, etc., and 220 family including phingomonadaceae, Gemmatimonadaceae, etc., and 329 genera including Sphingomonas, Rubrobacter, etc. Nine genera of nitrogen fixing bacteria were tested, the most high abundance of which were Arthrobacter, Methylobacterium belong to azotobacter, and Bradyrhizobium, Rhizobacter were relatively high abundance bacteria belong to rhizobia. [Conclusion] The bacterial diversity is abundant in the six samples from degraded grassland, but many species were at low abundance, perhaps they declined toward extinction.

Key words:High-throughput sequencing, Degradation, Azotobacter, Bacterial diversity