Identification of an efficient phosphorus solubilizing bacteria from saline-alkali soil in the Hexi Corridor and optimization of its condition

Abstract:

[Objective] In order to study the taxonomic status and characteristic of a highly effective phosphate-solubilizing strain Y3-35 which was isolated from the saline-alkali soil. [Methods] The morphological characteristics, physiological and biochemical characteristics, and 16S rRNA gene sequence of strain Y3-35 were analyzed. The method of halo ring was applied to isolate the phosphate-solubilizing bacteria, the method of Mo-Sb colorimetry was applied to measure phosphorus-dissolving ability. The single factor experiment and the orthogonal experiment were applied to find optimal phosphorus-dissolving condition of strain Y3-35. [Results] The result of identification revealed that phosphate-solubilizing strain Y3-35 can be classified as Pantoea aff. theicola. The phosphorus-dissolving ability of strain Y3-35 showed a negative correlation with pH. The result of the orthogonal experiment indicated that the optimal phosphorus-dissolving condition of phosphor bacteria Y3-35 was as follows: glucose 20.0 g/L, peptone 15.0 g/L, NaCl 2.5 g/L, 24 °C. The maximum phosphorus-dissolving ability could be up to 723.34 mg/L and the phosphorus-dissolving ability increased 251% than before. [Conclusion] The result indicates that Y3-35, an highly efficient phosphate-solubilizing bacteria, might act as a potential candidate for the application.