Determination of triterpenoids in Ganodema lingzhi from different areas and species by HPLC

Abstract:

[Objective] We established an HPLC (High Performance Liquid Chromatography) method to determine Ganoderic acid C2, Ganoderic acid B, Ganoderic acid A, Ganodermanontriol, Ganoderic acid DM, Ganoderic acid T, Ganoderiol B, Ganoderic acid S, Ganoderic acid G, Ganoderic acid F, Ganoderic acid D, Ganoderenic acid B in Ganodema lingzhi. [Methods] Aglient ZORBAX SB-Aq (4.6 mm×250 mm, 5 μm) was adopted as the chromatographic column, with acetonitrile-0.01% acetic acid solution using gradient elution at 30 oC with a flow rate of 1 mL/min, and the UV detection wavelength was set at 252 nm. Composition and content of different varieties of triterpenoids from different areas were determined by the above designed method. [Results] Triterpenoids in different varieties of Ganodema lingzhi in one area and samples from different areas were significantly varied in their compositions and contents; the method was sensitive, reliable and reproducible. [Conclusion] This method can be used to determine triterpenoids.

Key words:Ganodema lingzhi, HPLC, Triterpenoids