Identification and analysis of the microbe in Lidu ancient earthen cellar

Abstract:

[Objective] In this study, the microbial community structure of Lidu ancient earthen cellar was determined to provide reference for further microbial diseases prevention and protection of the earthen ruins. [Methods] We collected 3 representative samples from Lidu ancient earthen cellular to construct clone libraries of the 16S rRNA gene and 18S rRNA-ITS intergenic region and subsequently analyzed the sequencing results in order to establish phylogenetic tree by software such as MEGA 3.0 and ClustalW 1.8. [Results] A total of 72 16S rRNA gene and 80 18S rRNA-ITS intergenic sequences were obtained. All 16S rRNA gene sequences were classified into 3 phylum and 11 genera and all 18S rRNA-ITS intergenic sequences were classified into 1 phyla and 7 genera. The major bacteria included Halomonas sp., Methylohalomonas sp. and Lactobacillus sp. The major fungi included Cyphellophora sp., Pichia sp., Exophiala sp. and Phialophora sp. [Conclusion] Among the microbes we identified in this earthen ruins, dominant bacteria are slat-tolerant, which mainly caused by the earthen cellar’s environmental condition. Major fungus is filamentous fungi and yeast. It is important to identify the microbes which could destroy the earthen ruins, because the result provides scientific basis for the study of reinforcement material against these microorganisms and the protection of the Lidu ancient earthen cellar.

Key words:Lidu ancient earthen cellar, Microbial population, Reinforcement material, Biodegradability