Degradation of malachite green dye by Schizophyllum commune cfcc7252

Abstract:

[Objective] To evaluate the ability of Schizophyllum commune cfcc7252 in degrading Malachite Green (MG) dye. [Methods] Effect of oxygen demand, initial pH value, temperature, carbon source, nitrogen source, salinity and initial dye concentration on MG biodegradation by S. commune cfcc7252 was studied in flasks containing liquid cultures based on single factor experiment design. The toxicity of the biodegraded products of MG on plant seed germination and microbial growth was determined in petri dishes. [Results] Schizophyllum commune cfcc7252 could degrade MG under both aerobic and anaerobic conditions; 67.8% MG (initial concentration: 350 mg/L) was degraded under the culture condition of 10.0 g/L glucose, 5.0 g/L yeast extract, 0.01 mmol/L Zn2+ and pH 4.0 for 36 h. After continuous bleaching for 7 rounds, the degradation rate still reached over 95.4%. In addition, the degradation rate of S. commune cfcc7252 on MG was as high as 98% if the salinity of the culture was less than 10.20%. Toxicity test showed that the biodegraded products of MG were rarely toxic to plants of Vigna umbellate and Vigna unguiculata and microorganisms of Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa. [Conclusion] Schizophyllum commune cfcc7252 has strong potential to treat MG contaminated wastewater.