Characteristics of bacterial communities in rhizosphere and phyllosphere of wine grape plants

Abstract:

[Objective] To investigate the bacterial communities structure in rhizosphere and phyllosphere of different varieties of wine grapes. [Methods] By using samples of rhizosphere soil and phyllosphere leaf of different varities of wine grapes as the analysis materials, it was used the method of the terminal restriction fragment length polymorphism (T-RFLP) to study the microbial communities in the rhizosphere soil and phyllosphere, and analyzis the diversity of bacterial communities. [Results] The dominant microflora of wine grapes in rhizosphere soil is belonging to Proteobacteria, the dominant microflora of wine grapes in phyllosphere leaf is belonging to Actinomycetales. The order of Rhizosphere simpson index is Folle Blanche>Gewurztraminer>Chenin blanc>Semillon>Ugni Blanc>Muller-Thurgau>Sauvignon Blanc, Shannon index is keeping consistent with Simpson index. The largest of Simpson index in phyllosphere is Chenin blanc, the smallest is Muller-Thurgau, which is basically consistent with the index of Shannon. [Conclusion] the diversity indices of bacterial communities structure is keeping consistent with grape varitiesand, and the habitat is related closely to their colonization parts on plants.