Optimization of fermentation medium for a cold-adapted superoxide dismutase (SOD) produced by Lactobacillus plantarum sp. CLP0279

Abstract:

[Objective] In order to increase the activity of a cold-adapted superoxide dismutase (SOD) from Lactobacillus plantarum sp. CLP0279. [Methods] Based on the single-factor experiment, the fermentation medium for the production of SOD was optimized using Plackett-Burman method, Box-Behnken design and response surface analysis. [Results] The optimal fermentation medium (g/L) was determined as follows: corn meal powder 25.000, KH2PO4 2.600, K2HPO4 1.830, CuSO4·5H2O 0.011, ZnSO4·7H2O 0.014. Under these conditions, the maximum activity of SOD was 194.82 U/mL, which was 1.36 times comparing to that before optimization. [Conclusion] The activity of the cold-adapted SOD was obviously increased after optimization and three crucial factors (K2HPO4, CuSO4·5H2O and ZnSO4·7H2O) were verified by the response surface analysis, which might provide a foundation for the scale-up culture in the future.