Metarhizium solid-state fermentation optimization by response surface analysis

Abstract:

[Objective] In order to improve the yield and quality of the Metarhizium conidia spores, response surface design was used to optimize the medium components for solid fermentation of the Metarhizium anisopliae CY-1. [Methods] On the basis of one-factor experiment, medium components were optimized by the response surface design. [Results] The carbon and nitrogen sources were added to the best solid fermentation medium, which also contained cornmeal and rice husk (8:2, W/W), water ratio 1:0.8, 0.8% glucose, 2.5% ammonium sulfate, 0.8% KH2PO4. In the optimized solid fermentation medium, the theory of spores production was 7.45×109 CFU/g, and the validation was actually 6.94×109 CFU/g. [Conclusion] the response surface method was applied to optimize the components of solid fermentation medium of the M. anisopliae, and the spores powders were obtained, which laid a foundation for the research of the control the underground soil borne pathogens and insect pests and the research of the powder preparation and processing.

Key words:Metarhizium, Response surface, Optimization, Solid fermentation