Diversity of the culturable bacteria isolated from Aspongopus chinesis Dallas (Pantatomidae)

Abstract:

[Objective] To obtain an overview of the diversity of culturable bacteria inside the imago Aspongopus chinesis Dallas. [Methods] Pure culture, reverse transcription factor amplification (BOXA1R-PCR), 16S rRNA gene sequencing and phylogeny analysis were used to analyze the diversity of culturable bacteria inside the Aspongopus chinesis Dallas. Moreover, the antimicrobial activities, indole-3-acetic acid (IAA) production and amylase activities of the isolates were also monitored. [Results] Totally, 52 bacteria strains with different colony phenotype were isolated from six different culture media. Based on the colony phenotype and BOXA1R-PCR patterns, 12 typical strains were selected for 16S rRNA gene sequencing. According to the phylogeny analysis based on the 16S rRNA gene sequence, the isolates were affiliated to 4 genera including Bacillus, Pseudomonas, Stenotrophomonas and Burkholderia. Bacillus comprised the most dominant genus among all the isolates. 84.6% (44/52) of the isolates exhibited antimicrobial activities against the tested pathogens, 94.2% of the isolates showed abilities in producing IAA, and 43 strains (accounting for 82.7%) had amylase activities. [Conclusion] Diverse populations of bacteria were existed in the Aspongopus chinesis Dallas, and showed a potential application prospect.

Key words:Aspongopus chinesis Dallas, Culturable bacteria, Biodiversity, 16S rRNA gene