Biodiversity analysis of marine fungi producing extracellular fibrinolytic activity enzymes from the intertidal zone along Zhanjiang coast

Abstract:

[Objective] To investigate the biodiversity of marine fungi producing extracellular plasmin-like enzymes and plasminogen activators from the intertidal zone of Naozhou island and Xuwen coral reef nature reserve along Zhanjiang coast, and lay the foundation for discovery of new thrombolytics of plasmin-like enzymes and plasminogen activators. [Methods] Marine fungi were isolated and cultured by the method using potato dextrose agar (PDA) culture plates and yeast extract peptone dextrose (YPD) media, and identified by sequence analysis and phylogenetic tree construction of fungal rDNA internal transcribed spacer 1-5.8S rDNA-internal transcribed spacer 2 (ITS1-5.8S-ITS2) fragments. The marine fungi producing extracellular proteases were screening by the method using skim milk PDA (SM-PDA) plates, and the marine fungi producing extracellular plasmin-like enzymes and plasminogen activators were screening by the method using sea water fibrin PDA (FN-PDA) plates. [Results] A total of 446 strains of marine fungi were isolated, cultured and identified from the intertidal zone of Naozhou island and Xuwen coral reef nature reserve along Zhanjiang coast, which contained 98 species distributed in 65 genera, 46 families, 18 orders, 6 classes, belonged to Ascomycota and Basidiomycota of fungus. Amongst, there were 265 strains of marine fungi producing extracellular proteases represented 61 species distributed in 41 genera, 67 strains of fungi producing extracellular plasmin-like enzymes represented 22 species distributed in 14 genera, and 84 strains of fungi producing extracellular plasminogen activators represented 23 species distributed in 13 genera. The dominant genus was Aspergillus, and then Penicillium. [Conclusion] There were species biodiversity of the culturable marine fungi producing extracellular plasmin-like enzymes and plasminogen activators in the intertidal zone along Zhanjiang coast, which would be the rich resources for discovery of new thrombolytic agents.