Identification and sensitive drug test of pathogenic Proteus vulgaris from Rana catesbeiana

Abstract:

[Objective] Pathogen isolation, identification, and drug sensitivity test were performed on Rana catesbeiana that were suffering from skin ulcers in order to provide references to effectively prevent and control skin ulcers disease. [Methods] Pathogens, separated from the heart, liver and nidus of dying Rana catesbeiana infected by skin ulcers with the use of routine separation methods, were identified through artificial infection test, API 20E biochemical identification system, and 16S rRNA gene sequence analysis. Then, drug sensitivity test was conducted using paper diffusion method. [Results] One superior strain (NWG20141026), isolated from Rana catesbeiana infected by skin ulcers, exhibited strong pathogenicity to Rana catesbeiana by Artificial infection test. Through API 20E biochemical identification and 16S rRNA gene sequence analysis, it was found that the strain was Proteus vulgaris. The results from drug sensitivity test showed that phathogen of skin ulcer were highly sensitive to 7 medicines, such as florfenicol, tetracycline, doxycycline, nalidixic acid, sulfisoxazole, enrofloxacin, and erythrocin. [Conclusion] Skin ulcer disease, caused by Proteus vulgaris, could be treated by florfenicol, tetracycline, doxycycline, nalidixic acid, and sulfisoxazole.