Diversity of lactic acid bacteria associated with traditional fermented milk from Hulun Buir in Inner Mongolia

Abstract:

[Objective] The diversity of lactic acid bacteria in traditional fermented milk from Hulun Buir in Inner Mongolia was studied. [Methods] The samples were analyzed using pure culture and 16S rRNA gene sequence analysis. [Results] A total of 24 strains of lactic acid bacteria were obtained from eight fermented milk samples (6 fermented cow milk and 2 fermented mare milk). All the isolates were classified to 5 genera and 9 species: Lactobacillus kefiranofaciens (2 strains), Lactobacillus kefiri (2 strains), Lactobacillus paracasei (5 strains), Lactobacillus plantarum (3 strains), Lactobacillus rhamnosus (1 strain), Lactococcus lactis subsp. lactis (6 strains), Leuconostoc mesenteroides subsp. dextranicum (2 strains), Streptococcus thermophilus (2 strains), and Enterococcus faecium (1 strain). [Conclusion] Lactococcus lactis subsp. lactis is the dominant species, held 25% of the total isolated lactic acid bacteria, and Lactobacillus paracasei occupied 20.83% of the total lactic acid bacteria isolates as the predominant species of traditional fermented milk from Hulun Buir in Inner Mongolia.