Effect of soil physical and chemical properties on bacterial diversity in Tricholoma matsutake shiroes at harvest

Abstract:

[Objective] To investigate the effect of physical and chemical properties on the diversity of bacteria of Tricholoma matsutake shiroes. [Methods] Soil from Tricholoma matsutake shiroes and control treatments were collected at the T. matsutake’s harvest time in the main producing areas Xiaojin, Yajiang, Muli, Yanyuan, and Yanbian in Sichuan denaturing gradient gel electrophoresis (DGGE) was used to analyze the bacterial diversity in shiroes. Physical and chemical properties of soil samples were analyzed. Data analysis was done using principal component analysis (PCA) and multiple regression tree analysis (MRT). [Results] The physical and chemical properties of shiro soils were significantly different. The fertility level was significantly higher in shiro soils than in control treatments. The diversity indices in different counties were significantly different. However, bacterial diversity in the control soils was not necessarily lower than in shiro soils from the same area. MRT showed that higher organic matter (OM, 136.5 g/kg≤OM≤257.1 g/kg) and higher available nitrogen (AN, 151.2 mg/kg≤AN≤277.0 mg/kg) increased the bacterial diversity of shiro soils when the concentration of available copper (Cu) was higher than or equal to 0.296 7 mg/kg and less than 0.651 7 mg/kg. Whereas, lower pH (4.800