Progress in synthetic promoter library

Abstract:

With the development of synthetic biology, design and synthesis of functional elements show great potential in assisting and simplifying the study of metabolic engineering. The fundamental level of transcriptional control takes place at promoter elements that drive gene expression. Synthetic promoter library (SPL) provides promoter with different strength for fine-tuned gene expression. SPL is an important solution to optimal expression of gene and combinatorial optimization of metabolic pathways. This review is mainly focused on the current methods and advances in strategies of SPL. Apart from the description of the developments, we discuss the application of artificial promoter in metabolic engineering and research prospect of promoter engineering in the future.

Key words:Metabolic engineering, Synthetic biology, Promoter library, Transcriptional regulation, Combinatorial optimization