Functional analysis of a RND family efflux transporter component-cusB gene associated with copper resistance in Acidovorax citrulli

Abstract:

[Objective] we test the influence of RND family efflux transporter, MFP subunit cusB gene, on the copper resistance for Acidovorax citrulli. [Methods] Transposon (Tn5) was inserted into the genome randomly screened to prepare mutant strains constructed by functional complementation biparents mating and discussed on the effect of RND family efflux transporter MFP subunit cusB gene on the copper resistance for A. citrulli on basis of the copper resistance for A. citrulli, extracellular cellulase and extracellular protease secreted extracellular polysaccharide production, biofilm formation, virulence and motility etc. [Results] The mutant ΔcusB can’t be grown when it contained the 1.25 mmol/L CuSO4 or 2.5 mmol/L flat on KMB. The structure of cusB extracellular polysaccharide secretion and biofilm formation A. citrulli can be different from wild-type due to the effect of the mutant gene. Whatever, the mutant gene can’t directly affect the extracellular cellulase enzymes, extracellular protease, pathogenicity and allergic reactions also be obtained in our investigation. [Conclusion] The biological characteristics of A. citrulli can be affected by cusB associated mutations. Meanwhile, cusB associated mutations affect the copper resistance for A. citrulli.