Separation and identification of the main nutriments in Sporidiobolus pararoseus oil

Abstract:

[Objective] A new source of oil, produced by microorganism, was studied in the article. [Methods] Nine kinds of the main compositions were separated by twice slica column chromatography from the oil produced by Sporidiobolus pararoseus. The compositions were identified and quantized by thin layer chromatography, HPLC and HPLC-MS. [Results] The predominant fractions were squalene, β-carotene, γ-carotene, ergosterol esters, torulene, triglyceride, free fatty acids, ergosterol and torularhodin. The concentration of total ergosterol was about 3.2 g/kg in the Sporidiobolus pararoseus oil, and 16.7 % of the ergosterol exists in the form of ergosterol ester. The concentration of squalene in the oil was about 1.25 g/kg. The concentration of carotenoids in the oil was approximately 0.4 g/kg. Besides, the fatty acids compositions of ergosterol esters and triglyceride were also studied. There was about 73% oleic acid in the oil. [Conclusion] These results showed that S. pararoseus oil can be a good source of functional oil.

Key words:Sporidiobolus pararoseus oil, carotenoids, squalene, fatty acids