Characterization of chimeric foot-and-mouth disease viruses bearing the S-fragment of O/GSLX/CHN/2010

Abstract:

[Objective] We studied the characteristics of chimeric foot-and-mouth disease viruses (FMDVs) bearing the S-fragment of O/GSLX/CHN/2010. [Methods] Based on 2 existing full-length infectious cDNA clones with the same lineage to O/GSLX/CHN/2010, 2 chimeric FMDVs bearing the S-fragment of O/GSLX/CHN/2010 were constructed and rescued by reverse genetic manipulation. Virus replication and virulence in suckling mice of the chimeric viruses, parent genetically engineering viruses and FMDV O/GSLX/CHN/2010 were evaluated. [Results] FMDV O/GSLX/CHN/2010 replicated slowly and its virulence in suckling mice was weak. Whether in virus replication or virulence in suckling mice, the chimeric viruses and their parent genetically engineering viruses were better than FMDV O/GSLX/CHN/2010 significantly. [Conclusion] S-fragment of FMDV O/GSLX/CHN/2010 cannot determine the weak phenotype of O/GSLX/CHN/2010 alone, which lay the foundation for further studies of the attenuation mechanism of O/GSLX/CHN/2010 and the influence of S-fragment on FMDV characteristics.