The community structure of the intestine cultivable bacteria of Bactrocera dorsalis adults

Abstract:

[Objective] In order to identify the community structure of the cultivable bacteria inhabiting the intestinal tract of adult oriental fruit flies (Bactrocera dorsalis) from laboratory-reared, laboratory sterile sugar-reared, and field-collected populations. [Methods] 16S rRNA gene PCR-DGGE molecular methods was employed to study the bacterial diversity. At the same time, the common species of cultivable bacteria were identified by colony morphology, physiological and biochemical characteristics tests. [Results] Six hundred culturable bacteria in the intestinal tract of adult B. dorsalis from the aboved three populations were classified into 53 unique phylotypes. All sequenced bacteria strains were grouped into three families: Enterobacteriaceae, Enterococcaceae and Bacillaceae. The Enterobacteriaceae was dominated in all the populations. The closely related sequences (>97% sequence similarity) which had been retrieved from culturable bacteria of three populations were grouped as one common species. We determined the common species: five strains of bacteria as Enterobacter, two strains of Klebsiella, one strain of Citrobacter, one strain of Pantoea, two strains of Enterococcus, and four strains of Bacillus. [Conclusion] The present study significantly contributes to the available information on bacterial isolates and the biological control of B. dorsalis.

Key words:Bactrocera dorsalis, 16S rRNA gene, Intestine cultivable bacteria, DGGE, Community structure