Progress in DNA cloning and assembly techniques

Abstract:

DNA cloning and assembly techniques are essential tools for molecular biology research. With the recent advances in synthetic biology, efficient and fast assembly of large DNA including a number of genes is becoming more and more important. Meanwhile, a variety of DNA assembly methods are also developed very quickly. In this paper, various DNA assembly methods based on atypical enzyme digestion and ligation, PCR, homologous recombination, single strand annealing and splicing are summarized for providing effective technique tools for the further development of synthetic biology.

Key words:DNA cloning and assembly, Atypical enzyme digestion and ligation, PCR, Homologous recombination, Single strand annealing and splicing