Whole genome sequencing of a Micromonospora carbonacea strain with broad-spectrum antimicrobial activity

Abstract:

[Objective] Micromonospora carbonacea JXNU-1 is an actinomycete with broad-spectrum antimicrobial activity. This study aims to reveal its genomic sequence information. [Methods] The genomic DNA from M. carbonacea JXNU-1 was sequenced by Sanger technology using Illumina MiSeq and Illumina HiSeq 2500 sequencers. The genome was assembled by using SOAPdenovo software; multiplex PCR was used to close the gaps, and the genome sequence information was analyzed by bioinformatics methods. [Results] The whole-genomic DNA of M. carbonacea JXNU-1 was sequenced and annotated, and a fine genome map of M. carbonacea JXNU-1 was completed. This Whole Genome Shotgun project has been deposited at DDBJ/EMBL/GenBank under the accession JXSX00000000. [Conclusion] This study provides the basis to explore biosynthesis of antimicrobials from M. carbonacea JXNU-1.

Key words:Micromonospora carbonacea, Whole-genome sequencing, Biosynthetic gene cluster, Antimicrobial activity