Screening of microsatellite markers associated with acetic acid tolerance of Saccharomyces cerevisiae

Abstract:

[Objective] To screen microsatellite markers associated with acetic acid tolerance of Saccharomyces cerevisiae. [Methods] One hundred and sixty segregants were obtained by crossing two yeasts (high acetate tolerant YHA and low acetate tolerant YLA) with different phenotypes. The alleles of 15 microsatellite markers were amplified from 40 offspring yeasts and SPSS 11.5 software was used to analysis the relationship between acetic acid tolerance and microsatellite markers. [Results] Three microsatellite loci were identified. Among them marker 14P2 had significant positive correlation with acid tolerance (P<0.01), marker 15P2 and marker 15P3 had significant negative correlation with acid tolerance (P<0.01 and P<0.05). Furthermore, 70.6% of high tolerant individuals contained the marker 14P2 (344 bp) derived from YHA, the high tolerant strain; 91.3% of low tolerant individuals contained the marker 14P2 (331 bp) derived from YLA, the low tolerant strain. [Conclusion] This study indicated that the microsatellite marker 14P2 has the obvious tendency of heredity correlative with genes for acetic acid tolerance, it can be used in molecular marker assisted selection.

Key words:Microsatellite, Saccharomyces cerevisiae, Acetic acid tolerance, Marker assisted breeding