Bacterial community structure in the intestine of Exopalaemon carinicauda Holehuis

Abstract:

[Objective] To study bacterial communities and diversity in shrimp (Exopalaemon carinicauda Holehuis) intestine. [Methods] 16S rRNA gene was amplified and a library was constructed by genomic DNA that was extracted from the bacteria in shrimp intestine. [Results] Different RFLP patterns of the clones were obtained from analysis with Hae Ⅲ and Msp I. By comparing the obtained sequence with the published sequence in the GenBank database, 114 clones were obtained in the 16S rRNA clone library, and 11 different RFLP patterns of the clone were affirmed by Hae Ⅲ and Msp I. There were 8 clone sequences in high identity with known bacteria sequence, respectively belonging to Pseudomonas (17.5%), Enterobater (21.1%), Frigoribacterium (8.8%), Phaeobacter (5.3%), Vibrio (10.5%), Erythrobacter (9.6%), Aeromona (4.4%), and Staphylococcus (2.6%). In addition, uncultured bacteria accounted for 19.3%. [Conclusion] This study discovered the bacterial communities in the intestine of Exopalaemon carinicauda Holehuis, and the results would enhance our understanding about the bacterial community composition in Exopalaemon carinicauda Holehuis intestine and provide valuable data for the development of disease prevention mechanisms for shrimp cultivation.