Construction and characterization of the kdpD gene knock-out mutant of Vibrio alginolyticus
Author:
  • Article
  • | |
  • Metrics
  • | |
  • Related [20]
  • | | |
  • Comments
    Abstract:

    [Objective] To investigate the role of kdpD gene in Vibrio alginolyticus, a kdpD gene deletion mutant was generated. [Methods] Combining with chloramphenicol (Cm) and sucrose screening, we constructed an in-frame deletion mutant of kdpD using overlap PCR and homologous recombination technology. We then investigated the effect of the mutant on its growth, ECPase activity and virulence in fish. [Results] The mutant ΔkdpD was successfully constructed. The mutant ΔkdpD showed no differences of ECPase activity and growth from the wild-type strain. But a decrease in swarming ability and biofilm formation was found. Moreover, virulence assays with zebrafish model confirmed that virulence of the mutant declined by 8.84 times. [Conclusion] The kdpD gene could regulate swarming ability, biofilm formation and virulence, but it has no effect on growth and ECPase activity.

    Reference
    Cited by
    Comments
    Comments
    分享到微博
    Submit
Get Citation

ZHANG Yan-Fei, PANG Huan-Ying, JIAN Ji-Chang, LU Yi-Shan, WU Zao-He. Construction and characterization of the kdpD gene knock-out mutant of Vibrio alginolyticus[J]. Microbiology China, 2015, 42(9): 1770-1778

Copy
Share
Article Metrics
  • Abstract:1468
  • PDF: 2670
  • HTML: 554
  • Cited by: 0
History
  • Online: September 02,2015
Article QR Code