Isolation, identification and characterization of Neonothopanus nambi (Basidiomycota, Fungi), a new record from China

Abstract:

[Objective] A luminous Basidiomycete, designated MRC-lf3, was isolated from Fujian, China, and its taxonomic status was confirmed, and its life cycle was recorded. [Methods] The mycelial pure culture was obtained by tissue isolation method; morphological characteristics and the phylogenetic analysis of ITS rDNA sequence were used to analyze the taxonomic status of this fungus; the morphological and luminous characteristics were recorded by Digital Single Lens Reflex. [Results] Both morphological characteristics and ITS rDNA phylogenetic analysis results demonstrated that the strain MRC-lf3 was Neonothopanus nambi. Our study also finding that both ability to emit light and color of the fruiting body could be changed by different illumination intensity. [Conclusion] We isolated the luminous fungal strain from Fujian China, which was identified as Neonothopanus nambi, this is the ?rst record of genus Neonothopanus in China. This new record of Neonothopanus nambi brings the total number of luminous fungi in the Chinese Mainland to 8 species.

Key words:Luminescence, Basidiomycete, Morphology, ITS, Life cycle