Circular genetic information and biologic characteristics of mycobacteriophage Guo1

Abstract:

[Objective] To investigate the genetic and biologic characteristics of mycobacteriophage Guo1 with circular genome. [Methods] Sequenced the complete genome of Guo1 with Shot-gun library and config package strategy. Performed DNAStar, tRNAscan-SE, Promoter predictions, TRF, Glimmer, BLAST software for general genome characteristics, tRNA, promoter regions, tandem repeat sequences and putative function of predicted coding sequences, respectively. Utilized BLAST software to search for mycobacteriophages with genome similar as Guo1. Constructed phylogenetic tree to identify the cluster into which Guo1 should be grouped. Then measured the optimal multiplicity of infection and one-step growth curve, and observed the sensibility to ultraviolet, chloroform, alcohol, temperature, pH. [Results] Guo1 contains a double strand circular DNA with a full length of 40 086 nt, which belongs to the cluster G mycobacteriophage and which contains 59 putative genes, 7 promoter regions and three tandem repeat sequences. Guo1 is resistant to ultraviolet but sensitive to alcohol, chloroform and high temperature. The optimal temperature is 37 °C while the optimal pH is 7.0. The optimal MOI of Guo1 is about 0.01. One step growth of it showed that both latent period and burst period are 120 min, and the average burst size is about 47. [Conclusion] Guo1 is a cluster G mycobacteriophage with double strand circular DNA found for the first time. We sequenced the complete genome of Guo1 and identified the circular genetic information and biologic characteristics.