Research advances on yeast co-displaying multi-enzyme system in consolidated bioprocessing of cellulosic ethanol

Abstract:

Consolidated bioprocessing (CBP), combining cellulose production, saccharification, and fermentation into one step, has been proposed as the most efficient way to reduce the production cost of ethanol from cellulosic biomass. Based on Saccharomyces cerevisiae surface display technology, cellulases are displayed on the cell surface using two approaches (1) noncomplexed cellulases, (2) multicellulase complex (cellulosome). The assembly of cellulosome on Saccharomyces cerevisiae has becoming a new hot spot in cellulosic ethanol CBP research because of its high cellulose hydrolytic activity than noncomplexed cellulases. In this paper, the basic structure of cellulosome and their applications in cellulosic ethanol production are reviewed. Also, the prospects of this research field are given based on our analysis of the problems that still have not solved by the previous studies.