Identification of an inorganic phosphorus-dissolving bacterial strain BS06 and analysis on its phosphate solubilization ability

Abstract:

[Objective] The purpose of the present study was to investigate the phosphate-solubilizing ability of a bacterial stain BS06, for developing and applying effectively phosphate-solubilizing microbes in sugarcane production in Guangxi. [Methods] 16S rRNA gene sequence homology comparison, recA gene analysis as well as morphological, physiological and biochemical analyses were carried out to identify the bacterial strain BS06. The phosphate-solubilizing ability of BS06 was investigated under the conditions of changing the carbon and nitrogen source, separately in inorganic phosphate medium. [Results] The strain BS06 was identified as Burkholderia cepacia. The strain had high and efficient phosphate-solubilizing ability under the condition of carbon source with lactose and nitrogen source with sodium nitrate separately, and the concentration of water soluble phosphorus in fermentation solution was 262.71 mg/L and 305.85 mg/L, respectively. Compared with the uninoculated controls, BS06 significantly increased dry weights and phosphorus contents of micropropagated sugarcane seedlings. [Conclusion] The bacterial strain BS06 had high potential of exploitation.